Project 9: Development of an in-vitro animal model to evaluate novel pharmaceutical approached to DED management

Project Description

Barabino and Dana (2004) concluded “The existing animal models of dry eye mimic different pathogenic mechanisms of dry eye, but at the moment none of them seems to mirror precisely the complexity and chronicity of this frequent and debilitating condition.” The main disadvantage of most in-vitro models is that the experimental time frame is limited to only a few hours, which rules out any evaluation of epithelial defect healing.

This project will involve collaboration among engineers, biologists and health scientists to develop and test a complete anterior model for dry eye using porcine eyes, which are known to be similar in size and structure to the human eye. The ocular tissue will be perfused with a flowing, pressurised, temperature-regulated nutrient fluid to replicate the aqueous humour and the anterior ocular surface. It will be flushed at frequent intervals to simulate blinking-mediated tear film relocation.

Host Institution

Aston University (United Kingdom)